Obtaining sufficient genetic material from a limited biological source is currently the primary operational bottleneck in studies investigating biodiversity and genome evolution. A research team led by Dr. Isheng Jason Tsai at the Biodiversity Research Center, Academia Sinica has employed multiple displacement amplification (MDA) and Smartseq2 to amplify nanograms of genomic DNA and mRNA, respectively, from individual Caenorhabditis elegans. Through this research, the research team sampled and sequenced the genomes and transcriptomes of 13 nematodes from Dorylaimia, Enoplia, and early-branching species in Chromadoria. The result demonstrates the robustness of MDA in combination with ONT, paving the way for the study of genome diversity in the phylum Nematoda and beyond. This study has been published in in the journal Nucleic Acids Research (August 2023).
Article link: https://www.nature.com/articles/s41467-023-40406-7
Related link: https://abrc.sinica.edu.tw/en/view.php?aid=6589&wid=2