Marine ecology, phylogeography, genetic connectivity of marine metazoans.
Major Research Achievements (2013-2017)
Target taxon is not restricted but mainly invertebrate.
- Population subdivision and speciation are interesting and important evolutionary mechanism, which further generate biodiversity. Using individual- and community-based genetic analyses, we are demonstrating the importance of marginal seas for establishment of new populations and species in marine planktonic animals.
Designing of universal primer is critically important to recover community composition using community-based analyses. To achieve the task, we have identified conserved genetic region within mitochondrial 12S and nuclear 18S and 28S ribosomal RNA genes using database datasets. We now have sets of primers, which most likely recover most of metazoan species from any communities.
Global scale environmental problems, such as climate change and ocean acidification are emerging issues we are facing now. We need to understand not only the mechanism leading to those environment problems, but also the effect those problems have on organisms and ecosystems. We have established the community based genetic analysis (metagenetics and/or metagenomic) to estimate and describe diversity of marine animals. Using the technique, we are trying to understand distribution of marine animals in various temporal and spatial scales.
- Machida RJ, Leray M, Ho SL, Knowlton N, to appear, “Metazoan mitochondrial gene sequence reference datasets for taxonomic assignment of environmental samples”, Scientific Data, 4, 170027.
- Machida RJ, Lin YY, to appear, “Occurrence of Mitochondrial CO1 Pseudogenes in Neocalanus plumchrus (Crustacea: Copepoda): Hybridization Indicated by Recombined Nuclear Mitochondrial Pseudogenes”, PLOS ONE, 12(2), e0172710. (SCI) (IF: 3.057; SCI ranking: 17.5%)
- Fujioka HA, Machida RJ, Tsuda A , 2015, “Early life history of Neocalanus plumchrus (Calanoida: Copepoda) in the western subarctic Pacific ”, PROGRESS IN OCEANOGRAPHY, 137, 196-208. (SCI) (IF: 3.512; SCI ranking: 8.2%)
- Machida R.J. and Lin Y.Y. , 2014, “Four Methods of Preparing mRNA 5′ End Libraries Using the Illumina Sequencing Platform”, PLoS One, 9(7), e101812. (SCI) (IF: 3.057; SCI ranking: 17.5%)
- Miyamoto H, Machida RJ, Nishida S , 2012, “Global phylogeography of the deep-sea pelagic chetognath Eukrohnia hamata”, Progress in Oceanography, 104, 99-109. (SCI) (IF: 3.512; SCI ranking: 8.2%)
- Machida RJ*, Kweskin M, Knowlton N , 2012, “PCR primers for metazoan mitochondrial 12S ribosomal DNA sequences”, PLoS One, 7(4), e35887. (SCI) (IF: 3.057; SCI ranking: 17.5%)
- Machida, RJ*, Knowlton N , 2012, “PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences”, PLoS One, 7(9), e46180. (SCI) (IF: 3.057; SCI ranking: 17.5%)